ASTM E1218-04(2012)

1.1 This guide covers procedures for obtaining laboratory data concerning the adverse effects of a test material added to growth medium on growth of certain species of freshwater and saltwater microalgae during a static exposure. These procedures will probably be useful for conducting short-term toxicity tests with other species of algae, although modifications might be necessary. Although the test duration is comparable to an acute toxicity test with aquatic animals, an algal toxicity test of short duration (72, 96 or 120 h) allows for examination of effects upon multiple generations of an algal population and thus should not be viewed as an acute toxicity test.

1.2 Other modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparison of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting toxicity tests with microalgae.

1.3 These procedures are applicable to many chemicals, either individually or in formulations, commercial products, or known mixtures. With appropriate modifications, these procedures can be used to conduct tests on temperature, and pH and on such materials as aqueous effluents (see also Guide ), leachates, oils, particulate matter, sediments, and surface waters. Static tests might not be applicable to materials that are highly volatile, are rapidly biologically or chemically transformed in aqueous solutions, or are removed from test solutions in substantial quantities by the test vessels or organisms during the test. However, practical flow-through test procedures with microalgae have not been developed.

1.4 Results of tests using microalgae should usually be reported in terms of the 96-h (or other time period) IC50 (see ) based on reduction in growth. In some situations, it might only be necessary to determine whether a specific concentration unacceptably affects the growth of the test species or whether the IC50 is above or below a specific concentration.

1.5 This guide is arranged as follows:

	Section
Referenced Documents
Terminology
Summary of Guide
Significance and Use
Hazards
Apparatus
Facilities
Equipment
Test Vessels
Cleaning
Acceptability
Growth Medium
Test Material
General
Stock Solution
Test Concentration(s)
Test Organisms
Species
Source
Culture
Quality
Procedure
Experimental Design
Temperature
Product Details Published: 12/01/2012 Number of Pages: 14 File Size: 1 file , 180 KB Note: This product is unavailable in Russia, Ukraine, Belarus Document History ASTM E1218-04(2012) ASTM E1218-04e1 ASTM E1218-04 ASTM E1218-97a ASTM D7760-18 Standard Test Method for Measurement of Hydraulic Conductivity of Materials Derived from Scrap Tire.. $24.00 $48.00 Add to Cart ASTM D7414-18 Standard Test Method for Condition Monitoring of Oxidation in In-Service Petroleum and Hydrocarbon.. $32.00 $64.00 Add to Cart ASTM C1527/C1527M-11(2018) Standard Specification for Travertine Dimension Stone.. $21.00 $42.00 Add to Cart ASTM E950/E950M-09(2018) Standard Test Method for Measuring the Longitudinal Profile of Traveled Surfaces with an Accelerom.. $34.00 $68.00 Add to Cart